Ultra-LEAF™ Purified anti-α-E-Catenin
- 产品名称：
- Ultra-LEAF™ Purified anti-α-E-Catenin
- 产品类别：
- 抗体
- 产品编号：
- 844803
- 产品应用：
- 844803
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human MBP-alpha catenin fusion protein.
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
ICC - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. For Western blotting, the suggested use of this reagent is 5.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Sacco PA, et al. 1995. J. Biol. Chem. 270:20201. (WB, IP, IF)
- Johnson KR, et al. 1993. Exp. Cell Res. 2:252.
- RRID
- AB_2565809 (BioLegend Cat. No. 844803)
- Structure
- A 102 kD member of the α-Catenin family composed primarily of alpha helical bundles. It contains three vinculin homology domains, a vinculin binding region, an actin binding domain, and a dimerization domain.
- Distribution
-
Cytoplasm, cytoskeleton, cell junctions, adherens junctions, cell membrane, peripheral membraine protein, and the cytoplasmic side.
- Function
- Involved in endothelial cell survival, migration, contact-dependent growth inhibition, hemophilic adhesion, signal transduction, Wnt signaling, and cell junction organization.
- Interaction
- α-catenin binds to β-catenin. Cadherin binds to the p120-catenin and β-catenin. This cadherin-catenin complex interacts with the actin cytoskeleton. α-catenin can also bind vinculin, then associate with actin nucleating proteins.
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Hirano S, et al. 2012. Physiol. Rev. 92:597.
- Gene ID
- 1495 View all products for this Gene ID
- UniProt
- View information about alpha-E-Catenin on UniProt.org
