PerCP/Cyanine5.5 anti-rat CD3 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-rat CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 201417
- 产品应用：
- 201417
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- F344 rat spleen cells stimulated with PMA and calcium ionophore
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.?
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2.
- Application References
(PubMed link indicates BioLegend citation) -
- Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP)
- Nicholls MR, et al. 1993. Transplantation 55:459. (Block)
- Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF)
- Baba T, et al. 2006. Blood 107:2004. (IHC)
- Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P)
- Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed
- RRID
- AB_2860753 (BioLegend Cat. No. 201417) AB_2860754 (BioLegend Cat. No. 201418)
- Structure
- Ig superfamily, approximately 20-25 kD
- Distribution
-
Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
- Function
- Antigen recognition, TCR signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen/MHC complex
- Cell Type
- NKT cells, T cells, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.
- Gene ID
- 25710 View all products for this Gene ID 300678 View all products for this Gene ID 315609 View all products for this Gene ID 25300 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
