Purified anti-Cytokeratin 8 Antibody
- 产品名称：
- Purified anti-Cytokeratin 8 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 904804
- 产品应用：
- 904804
- Verified Reactivity
- Human, Mouse
- Reported Reactivity
- Dog, Non-Human Primate
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against the 12 carboxy-terminal amino acids of HK-8 preceded by a cysteine, CKLVSESSDVLPK.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a concentration range of 0.5 - 5.0 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy4.
- Application References
(PubMed link indicates BioLegend citation) -
- Memarzadeh S, et al. 2010. Proc. Natl. Acad. Sci. USA 107:17298. PubMed
- Hembrough TA. 1996. J. Biol. Chem. 271:25684. PubMed
- Hübner A, et al. 2012. Proc. Natl. Acad. Sci. USA 109:12046. PubMed
- Easter SL, et al. 2014. PLoS One 9(11):e113247. (IF) PubMed
- Product Citations
-
- Fujimori S, et al. 2022. Elife. 11:. PubMed
- Amonkar GM, et al. 2022. STAR Protoc. 3:101390. PubMed
- Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed
- Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
- Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
- Mao X, et al. 2022. Int J Mol Sci. 23:. PubMed
- RRID
- AB_2616821 (BioLegend Cat. No. 904804)
- Structure
- Cytokeratin 8 is a 483 amino acid protein with a molecular mass of 54 kD.
- Distribution
-
Tissue distribution: colon, small intestine, pancreas, liver, stomach, and salivary glands.
Cellular distribution: extracellular and nucleus.
- Function
- Cytokeratin 8 plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Together with KRT19, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Intermediate Filaments
- Gene ID
- 3856 View all products for this Gene ID
- UniProt
- View information about Cytokeratin 8 on UniProt.org
