APC anti-human C5L2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2136	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications include: Western blotting^1,3 and blocking^2,3. The LEAF? or?Ultra-LEAF? purified antibody is recommended for functional assays (contact our custom solutions team).

Clone 1D9-M12 is known to block C5a binding to C5L2, but does not react with C5a receptor. It has been reported that this clone is not effective in immunohistochemistry of formalin-fixed paraffin-embedded sections¹.

Application References (PubMed link indicates BioLegend citation)

Fonseca MI, et al. 2013. J. Neuroinflammation. 10:25. (WB) PubMed
Hao J, et al. 2013. PLoS One 8:e66305. (Block)
Raby AC, et al. 2011. Eur. J. Immunol. 41:2741. (Block, WB)

Product Citations

Zeng W, et al. 2022. Oncogene. Online ahead of print. PubMed

RRID

AB_2564330 (BioLegend Cat. No. 342405) AB_2564331 (BioLegend Cat. No. 342406)

Antigen Details

Structure: Seven transmembrane protein, decoy receptor, does not couple to G-proteins.
Distribution: Neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes.
Function: Negative regulation of C5a function.
Ligand/Receptor: C5a, C5a-des-Arg.
Cell Type: Astrocytes, Dendritic cells, Macrophages, Mast cells, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Scola AM, et al. 2009. Mol. Immunol. 46:1149
2. Johswich K, et al. 2006. J. Bio. Chem. 281:39088
3. Gerad NP, et al. 2005. J. Bio. Chem. 280:39677
4. Okinaga S, et al. 2003. Biochemistry. 42:9406
Gene ID: 27202 View all products for this Gene ID
UniProt: View information about C5L2 on UniProt.org

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