PerCP/Cyanine5.5 anti-mouse CD19 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD19 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 152405
- 产品应用：
- 152405
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Transfected k562 cells expressing the extracellular domain of murine CD19.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
- Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
- Product Citations
-
- Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
- Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
- Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
- Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
- Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
- Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
- Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
- Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
- Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
- Dai G, et al. 2021. mSphere. 6: . PubMed
- Ronsard L, et al. 2021. Front Immunol. 12:730471. PubMed
- Takahashi F, et al. 2022. iScience. 25:104278. PubMed
- McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
- Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
- Monzon-Casanova E, et al. 2021. Eur J Immunol. 51:2266. PubMed
- Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed
- Lee A, et al. 2022. Nat Commun. 13:549. PubMed
- Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
- RRID
- AB_2629814 (BioLegend Cat. No. 152405) AB_2629815 (BioLegend Cat. No. 152406)
- Structure
- Ig superfamily, associates with CD21 and CD81, 95 kD.
- Distribution
-
Pro-B cells to mature B cells (during development), follicular dendritic cells.
- Function
- Modulates B cell activation and differentiation.
- Ligand/Receptor
- CD21, CD81, Leu-13.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
- Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
