Pacific Blue™ anti-mouse CD19 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1128	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Transfected k562 cells expressing the extracellular domain of murine CD19.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.?

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: blocking^1,2 and immunoprecipitation¹.

Application References (PubMed link indicates BioLegend citation)

Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)

RRID

AB_2927869 (BioLegend Cat. No. 152415) AB_2927869 (BioLegend Cat. No. 152416)

Antigen Details

Structure: Ig superfamily, associates with CD21 and CD81, 95 kD.
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells.
Function: Modulates B cell activation and differentiation.
Ligand/Receptor: CD21, CD81, Leu-13.
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

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