Ultra-LEAF™ Purified anti-human CD314 (NKG2D-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2184	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IP, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation^1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation^2-7. For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered.

Application References (PubMed link indicates BioLegend citation)

Wu J, et al. 1999. Science 285:730.
Wu J, et al. 2000. J. Exp. Med. 192:1059.
Groh V, et al. 2001. Nature Immunol. 2:255.
Wu J, et al. 2002. J. Immunol. 169:1236.
Roberts A, et al. 2001. J. Immunol. 167:5527.
Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Watanbe M, et al. 2014. Int Immunol. PubMed

Product Citations

Breman E, et al. 2019. Front Immunol. 9:2940. PubMed
Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed
Dudek M, et al. 2021. Nature. 592:444. PubMed
Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed
Glasner A, et al. 2017. Sci Rep. 7:40944. PubMed

RRID

AB_2810480 (BioLegend Cat. No. 320813) AB_2561488 (BioLegend Cat. No. 320814)

Antigen Details

Structure: C-type lectin
Distribution: NK cells, γ/δ T cells, CD8⁺ T cells
Function: Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
Ligand/Receptor: MICA, MICB, UL16-binding proteins (ULBPs)
Cell Type: NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Jamieson AM, et al. 2002. Immunity 17:19.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.
7. Maasho K, et al. 2005. J. Immunol. 174:4480.
8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
Gene ID: 22914 View all products for this Gene ID
UniProt: View information about CD314 on UniProt.org

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