PE/Dazzle™ 594 anti-human CD314 (NKG2D) Anti-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1632	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation^1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation^2-7. For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered.

Application References (PubMed link indicates BioLegend citation)

Wu J, et al. 1999. Science 285:730.
Wu J, et al. 2000. J. Exp. Med. 192:1059.
Groh V, et al. 2001. Nature Immunol. 2:255.
Wu J, et al. 2002. J. Immunol. 169:1236.
Roberts A, et al. 2001. J. Immunol. 167:5527.
Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Watanbe M, et al. 2014. Int Immunol. PubMed

Product Citations

de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
Fernandez IZ, et al. 2019. J Exp Med. 216:1255. PubMed

RRID

AB_2687172 (BioLegend Cat. No. 320827) AB_2687173 (BioLegend Cat. No. 320828)

Antigen Details

Structure: C-type lectin
Distribution: NK cells, γ/δ T cells, CD8⁺ T cells
Function: Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
Ligand/Receptor: MICA, MICB, UL16-binding proteins (ULBPs)
Cell Type: NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Jamieson AM, et al. 2002. Immunity 17:19.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.
7. Maasho K, et al. 2005. J. Immunol. 174:4480.
8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
Gene ID: 22914 View all products for this Gene ID
UniProt: View information about CD314 on UniProt.org

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