Ultra-LEAF™ Purified anti-mouse CD40 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2112	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Costim, Block, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 1C10 antibody has been reported to stimulate B cell proliferation and block CD40-CD40L interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking^5,6 of CD40 ligand binding, and in vitro stimulation of B cell proliferation^1-3. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 102810). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 102812) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Ferlin WG, et al. 1998. Eur. J. Immunol. 28:525. (FC, Costim)
Ferlin WG, et al. 1996. Eur. J. Immunol. 26:2911. (Costim)
Heath A, et al. 1994. Eur. J. Immunol. 24:1828. (Costim)
Ashman RF, et al. 2005. Int. Immunol. 17:411.
Pel?ez B, et al. 2001. J. Immunol. 166:6608. (Block)
Zhang P, et al. 2007. J. Immunol. 178:474. (Block)
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Ponomarev ED, et al. 2007. J.Immunol.178:39. PubMed

RRID

AB_2783043 (BioLegend Cat. No. 102811) AB_2561489 (BioLegend Cat. No. 102812)

Antigen Details

Structure: TNFR superfamily, 48 kD
Distribution: B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34⁺ hematopoietic progenitors
Function: Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
Ligand/Receptor: CD40L (CD154)
Cell Type: B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
Gene ID: 21939 View all products for this Gene ID
UniProt: View information about CD40 on UniProt.org

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