Biotin anti-mouse CD43 Activation-Associated Glyco-上海雅吉生物科技有限公司

规格	价格	库存
200µg	￥ 2748	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse WEHI 274.3 myeloid tumor cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, Western blotting^1,2, and immunohistochemistry of acetone-fixed frozen sections.

Application References (PubMed link indicates BioLegend citation)

Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
Onami TM, et al. 2002. J. Immunol. 168:6022.
van der Most RG, et al. 2003. Intl. Immunol. 15:119.
Chu VT, et al. 2007. J. Immunol. 179:5947.
Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed

Product Citations

Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
Chu V, et al. 2007. J Immunol. 179:5947. PubMed
Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
Hu K, Butte M 2016. J Cell Biol. 213: 535 - 542. PubMed

RRID

AB_493384 (BioLegend Cat. No. 121204)

Antigen Details

Structure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation
Distribution: Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5⁺ B-1 cells and activated B cells, and hemotopoietic progenitor cells.
Function: Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor: CD54, Siglec-1
Cell Type: B cells, Granulocytes, Hematopoietic stem and progenitors, Monocytes, NK cells, Plasma cells, Platelets, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID: 20737 View all products for this Gene ID
UniProt: View information about CD43 on UniProt.org

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