Alexa Fluor® 647 anti-mouse CD43 Activation-As-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2748	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse WEHI 274.3 myeloid tumor cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume.?For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, Western blotting^1,2, and immunohistochemistry of acetone-fixed frozen sections.

Application References (PubMed link indicates BioLegend citation)

Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
Onami TM, et al. 2002. J. Immunol. 168:6022.
van der Most RG, et al. 2003. Intl. Immunol. 15:119.
Chu VT, et al. 2007. J. Immunol. 179:5947.
Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed

Product Citations

Venosa A, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L1487 - L1498. PubMed
Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed

RRID

AB_528804 (BioLegend Cat. No. 121212)

Antigen Details

Structure: Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation
Distribution: Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5⁺ B-1 cells and activated B cells, and hemotopoietic progenitor cells.
Function: Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand/Receptor: CD54, Siglec-1
Cell Type: B cells, Granulocytes, Hematopoietic stem and progenitors, Monocytes, NK cells, Plasma cells, Platelets, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID: 20737 View all products for this Gene ID
UniProt: View information about CD43 on UniProt.org

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