Ultra-LEAF™ Purified anti-mouse CD210 (IL-10-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Soluble mIL-10R-FLAG-His

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding^1-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 112706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112704) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 112710) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

O'Farrell A, et al. 1998. EMBO J. 17:1006. (Block)
Wang W, et al. 2004. FASEB J. 18:1043. (Block)
Vieira PL, et al. 2004. J. Immunol. 172:5986. (Block)
Iwashiro M, et al. 2001. P. Natl. Acad. Sci. USA 98:9226. (Block)
Vicari AP, et al. 2002. J. Exp. Med. 196:541. (Block)

Product Citations

Gottschalk RA, et al. 2019. Elife. 8:e46836. PubMed
Kuan Y, et al. 2013. PLoS One. 8:72422. PubMed
Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
Murdock B, et al. 2014. J Immunol. 193:4107. PubMed

RRID

AB_11147950 (BioLegend Cat. No. 112709) AB_11149684 (BioLegend Cat. No. 112710) AB_2616679 (BioLegend Cat. No. 112711) AB_2616680 (BioLegend Cat. No. 112712) AB_2832377 (BioLegend Cat. No. 112713) AB_2832378 (BioLegend Cat. No. 112714)

Antigen Details

Structure: Class II cytokine receptor family, 90-110 kD
Distribution: Thymocytes, T and B cells, NK cells, monocytes, macrophages
Function: Inhibits cytokine production, costimulates B cell proliferation and differentiation
Ligand/Receptor: IL-10
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Ho A, et al. 1993. P. Natl. Acad. Sci. USA 90:11267.
2. Tan JC, et al. 1993. J. Biol. Chem. 268:21053.
3. Niemand C, et al. 2003. J. Immunol. 170:3263.
4. Corinti S, et al. 2001. J. Immunol. 166:4312.
Gene ID: 16154 View all products for this Gene ID
UniProt: View information about CD210 on UniProt.org

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