Purified anti-Histone H3 (C-terminus) Antibody
- 产品名称：
- Purified anti-Histone H3 (C-terminus) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 819411
- 产品应用：
- 819411
- Verified Reactivity
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
- Formulation
- 50 mM sodium citrate, 150mM sodium chloride, pH 5.8 with stabilizer
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P -?Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration of 1.0 ?g/mL is suggested. For Western blotting, the suggested use of this reagent is 0.01 - 0.5 ?g/mL.?It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Reactivity to Drosophila was only verified with the purified format.
- Additional Product Notes
For Western blotting, the suggested use of this reagent is 0.01 - 0.2 ?g/mL in human, mouse, and rat and 0.1 - 0.5 ?g/mL in Drosophila.
- Product Citations
-
- Bado IL, et al. 2021. Developmental Cell. 56(8):1100-1117.e9. PubMed
- RRID
- AB_2820127 (BioLegend Cat. No. 819411) AB_2820128 (BioLegend Cat. No. 819412)
- Structure
- Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
- Distribution
-
Nucleus
- Function
- Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
- Interaction
- Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
- Antigen References
-
1. Chen HM,?et al.?2015.?Epigenetics and Dermatology?409.
- Gene ID
- 8350 View all products for this Gene ID
- UniProt
- View information about Histone H3 C-terminus on UniProt.org
