Go-ChIP-Grade™ Purified anti-Histone H3 (C-t
- 产品名称：
- Go-ChIP-Grade™ Purified anti-Histone H3 (C-t
- 产品类别：
- 抗体
- 产品编号：
- 819413
- 产品应用：
- 819413
- Verified Reactivity
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide with stabilizer
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
IHC-P, WB - Verified
- Recommended Usage
The recommended starting antibody to chromatin ratio is 1 ?g of antibody and 5 ?g of chromatin per IP. Optimal antibody to chromatin ratio should be determined by the end user. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 1.0 ?g/mL is suggested.?For western blotting, the suggested use of this reagent is 0.01 - 0.5 ?g/mL. It is recommended that the reagent to be titrated for optimal performance before each experiment.
- Application Notes
Reactivity to Drosophila was only verified with the purified format.
- RRID
- AB_2832856 (BioLegend Cat. No. 819413) AB_2832857 (BioLegend Cat. No. 819414)
- Structure
- Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
- Distribution
-
Nucleus
- Function
- Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
- Interaction
- Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
- Antigen References
-
1. Chen HM,?et al.?2015.?Epigenetics and Dermatology?409.
- Gene ID
- 8350 View all products for this Gene ID
- UniProt
- View information about Histone H3 C-terminus on UniProt.org
