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Ultra-LEAF™ Purified anti-mouse CD1d (CD1.1,
产品名称:
Ultra-LEAF™ Purified anti-mouse CD1d (CD1.1,
产品类别:
抗体
产品编号:
123531
产品应用:
123531
[价格]
规格 价格 库存
100µg ¥ 1980 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma
Formulation
0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC, IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistical staining, and blocking function3.
?

This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block)
  2. Brozovic S, et al. 2004. Nat. Med. 10:535.
  3. Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block)
  4. Jiang J, et al. 2012. PLoS One. 7:47487. PubMed
RRID
AB_2832442 (BioLegend Cat. No. 123531) AB_2832443 (BioLegend Cat. No. 123532)

Antigen Details

Structure
48 kD glycoprotein, Ig superfamily
Distribution

leukocytes, dendritic cells, epithelial cells, thymocytes

Function
Antigen presentation
Ligand/Receptor
similar to MHC class I, associate with ?-microglobulin
Cell Type
Dendritic cells, Epithelial cells, Leukocytes, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Brudin N, et al. 1998. J. Immunol. 161:3271.
2. Amano M, et al. 1998. J. Immunol. 161:1710.
3. Brossay L, et al. 1997. J. Immunol. 159:1216.
4. Dougan SK, et al. 2007. Curr. Top. Microbiol. Immunol. 314:113.
5. Brutkiewicz RR, et al. 2007. Eur. J. Immunol. 37:2390.

Gene ID
12479 View all products for this Gene ID
UniProt
View information about CD1d on UniProt.org

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