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Purified anti-SARS-CoV S Protein S2 Antibody
产品名称:
Purified anti-SARS-CoV S Protein S2 Antibody
产品类别:
抗体
产品编号:
944301
产品应用:
944301
[价格]
规格 价格 库存
25µg ¥ 2052 1

产品详情

Product Details

Verified Reactivity
SARS-CoV-2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant SARS-CoV protein corresponding to region of S2 subunit
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ELISA - Verified
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0125?- 0.1?g/mL.?For ELISA?applications, a concentration of 30.4 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

BioLegend offers two clones, 1A9/S2 and A20085C, that recognize SARS-CoV-2 S protein S2.

Both clones have been verified for western blot and direct ELISA, but clone 1A9/S2 is considerably more sensitive for both applications.

Clone 1A9/S2 has been reported to show reactivity towards SARS-CoV (Ng OW, et al. 2014. PLoS One. 9:e102415; Lip KM, et al. 2006. J?Virol. 80:941-950.)

Application References

(PubMed link indicates BioLegend citation)
  1. Ng?OW, et al. 2014. PLoS One. 9:e102415. (WB, Block)
  2. Lip KM, et al. 2006. J Virol. 80:941-950. (Block)
RRID
AB_2890871 (BioLegend Cat. No. 944301) AB_2890871 (BioLegend Cat. No. 944302)

Antigen Details

Structure
Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 amino acids with a theoretical molecular weight of 141 kD, and consists of S1 and S2 subunits. The S2 domain is 587 amino acids long with a predicted molecular weight of 64 kD.
Distribution

Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane

Function
Mediates fusion between virus and host cell membranes
Interaction
ACE2
Ligand/Receptor
ACE2
Biology Area
COVID-19
Antigen References
  1. Walls AC, et al. 2020. Cell. 181:281-292.
  2. Yan R, et al. 2020. Science. 367:1444-1448.
  3. Wrapp D, et al. 2020. Science. 367:1260-1263.
  4. Shang J, et al. 2020. Proc Natl Acad Sci U S A. 117:11727-11734.
Gene ID
43740568 View all products for this Gene ID
UniProt
View information about Viral Protein on UniProt.org

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