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首页 > 抗体蛋白 > 抗体
Ultra-LEAF™ Purified anti-mouse Ly-6G Antibo
产品名称：
Ultra-LEAF™ Purified anti-mouse Ly-6G Antibo
产品类别：
抗体
产品编号：
127632
产品应用：
127632
[价格]
规格 价格 库存
1mg ￥ 1788 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Depletion, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo?studies or highly sensitive assays (Cat. No.?127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  21. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Haffner–Luntzer M, et al. 2019. Proc Natl Acad Sci U S A. 116:8615. PubMed
  2. Gawish R, et al. 2022. Elife. 11:. PubMed
  3. Deng H, et al. 2021. J Immunother Cancer. 9: . PubMed
  4. Prystaz K, et al. 2018. Am J Pathol. 188:474. PubMed
  5. M?dinger Y, et al. 2018. Am J Pathol. 189:147. PubMed
  6. Kaiser R, et al. 2021. JCI Insight. 6:e150862. PubMed
  7. Kuhn MR, et al. 2022. Front Endocrinol (Lausanne). 13:997745. PubMed
  8. Hu J, et al. 2022. J Immunol Res. 2022:7966089. PubMed
  9. Pan YC, et al. 2021. Mol Ther Oncolytics. 20:175. PubMed
  10. Shang Q, et al. 2020. Cell Death Dis. 0.949305556. PubMed
  11. Kim AD, et al. 2021. Sci Rep. 11:24194. PubMed
  12. Fischer V, et al. 2022. J Bone Miner Res. 37:137. PubMed
  13. Bergdolt S, et al. 2017. PLoS One. 12(6):e0179512. PubMed
  14. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  15. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  16. Zheng Z, et al. 2021. Nat Commun. 12:6202. PubMed
  17. Chen J, et al. 2021. J Invest Dermatol. 787:141. PubMed
  18. Calvente CJ, et al. 2019. J Clin Invest. 130:4091. PubMed
  19. Kaiser K, et al. 2018. Naunyn Schmiedebergs Arch Pharmacol. 391:523. PubMed
  20. Kemmler J, et al. 2016. PLoS One. 10: 0131194. PubMed
RRID
AB_11150581 (BioLegend Cat. No. 127632) AB_11150581 (BioLegend Cat. No. 127680) AB_2572001 (BioLegend Cat. No. 127649) AB_2572002 (BioLegend Cat. No. 127650) AB_2860668 (BioLegend Cat. No. 127661) AB_2860669 (BioLegend Cat. No. 127662)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

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