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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse Ly-6G Antibody
产品名称：
Pacific Blue™ anti-mouse Ly-6G Antibody
产品类别：
抗体
产品编号：
127611
产品应用：
127611
[价格]
规格 价格 库存
25µg ￥ 1260 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo?studies or highly sensitive assays (Cat. No.?127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  21. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
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  2. Huang W, et al. 2015. J Allergy Clin Immunol. . PubMed
  3. Barkaway A, et al. 2021. Immunity. . PubMed
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  5. Honda M, et al. 2021. Nat Commun. 12:7294. PubMed
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  12. Her Z, et al. 2015. EMBO Mol Med. 7:24. PubMed
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  14. Cardona S, et al. 2015. ASN Neuro. 7: 1759091415608204. PubMed
  15. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
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  19. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  20. Chew W, et al. 2016. Nat Methods. 13: 868-874. PubMed
  21. McCreedy DA et al. 2018. eNeuro. 5(4) pii: ENEURO. PubMed
  22. Gibbons M, et al. 2011. Am J Respir Crit Care Med. 184:569. PubMed
  23. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  24. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  25. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
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  27. Wessel AW, et al. 2020. Nat Commun. 4.123611111. PubMed
  28. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  29. Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
  30. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  31. Dorward D, et al. 2013. J Leukoc Biol. 94:193. PubMed
  32. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  33. Gittens B, et al. 2017. J Immunol. 10.4049/jimmunol.1600709. PubMed
  34. Arnardottir H, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  35. Colombo M, et al. 2022. iScience. 25:105042. PubMed
  36. Zhang L, et al. 2015. J Immunol. 194:5423. PubMed
  37. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  38. Dong L, et al. 2022. Cells. 11:. PubMed
  39. Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed
  40. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  41. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  42. Kim K, et al. 2014. Proc Natl Acad Sci U S A. 111:11774. PubMed
  43. Wang B, et al. 2015. Sci Rep. 5: 17695. PubMed
  44. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  45. Teo T, et al. 2015. J Virol. 89: 7955-7969. PubMed
  46. Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
  47. Hickey A, et al. 2013. Infect Immun . 81:2123. PubMed
  48. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  49. Myers DR, et al. 2020. Front Immunol. 11:576310. PubMed
  50. Kumari P, et al. 2021. Cell Reports. 35(3):109012. PubMed
  51. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  52. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  53. de Gaetano M, et al. 2021. J Med Chem. 64:9193. PubMed
  54. Avila M, et al. 2012. Innate Immunity. 18:648. PubMed
  55. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  56. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
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RRID
AB_1877212 (BioLegend Cat. No. 127611) AB_2251161 (BioLegend Cat. No. 127612)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

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