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Brilliant Violet 650™ anti-mouse Ly-6G Antib
产品名称：
Brilliant Violet 650™ anti-mouse Ly-6G Antib
产品类别：
抗体
产品编号：
127641
产品应用：
127641
[价格]
规格 价格 库存
50µg ￥ 3564 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo?studies or highly sensitive assays (Cat. No.?127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  21. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  2. Hu K, et al. 2022. J Extracell Vesicles. 11:e12199. PubMed
  3. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  4. Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed
  5. Borkner L, et al. 2021. Mucosal Immunol. 14:1183. PubMed
  6. Kim YS, et al. 2021. Cell Death Dis. 12:307. PubMed
  7. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  8. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  9. Fujii Y, et al. 2022. JBMR Plus. 6:e10562. PubMed
  10. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  11. Moreau GB, et al. 2020. Am J Trop Med Hyg. 103:1215. PubMed
  12. Zong F, et al. 2022. Front Immunol. 13:900755. PubMed
  13. Yu S, et al. 2016. Mol Ther. 10.1038/mt.2016.175. PubMed
  14. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  15. Wang ECE, et al. 2019. Cell Stem Cell. 24:654. PubMed
  16. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  17. DiPiazza A, et al. 2017. Sci Rep. 10.1038/s41598-017-11313-x. PubMed
  18. Fujii T, et al. 2021. Bone Res. 9:4. PubMed
  19. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  20. Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
  21. Sung PS, et al. 2022. JCI Insight. :. PubMed
  22. Yun H, et al. 2020. Immunity. 53(5):1050-1062.e5. PubMed
  23. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  24. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  25. Donlan AN, et al. 2021. JCI Insight. 6: . PubMed
  26. Poillet–Perez L, et al. 2018. Nature. 563:569. PubMed
  27. Tao Z, et al. 2022. Cells. 11:. PubMed
  28. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  29. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
RRID
AB_2565881 (BioLegend Cat. No. 127641)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

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