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Brilliant Violet 510™ anti-mouse Ly-6G Antib
产品名称：
Brilliant Violet 510™ anti-mouse Ly-6G Antib
产品类别：
抗体
产品编号：
127633
产品应用：
127633
[价格]
规格 价格 库存
50µg ￥ 3564 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo?studies or highly sensitive assays (Cat. No.?127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  21. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  2. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  3. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  4. Bertocchi A, et al. 2021. Cancer Cell. 39(5):708-724.e11. PubMed
  5. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  6. Amor C, et al. 2020. Nature. 583:127. PubMed
  7. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  8. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  9. Eisele AS, et al. 2022. Elife. 11:. PubMed
  10. Cassidy BR, et al. 2020. J Neuroinflammation. 17:259. PubMed
  11. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  12. Salvioni A et al. 2019. Cell Rep. 27(11):3254-3268 . PubMed
  13. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  14. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  15. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  16. Kamp M, et al. 2016. PLoS One. 11: 0163750. PubMed
  17. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  18. Uribesalgo I, et al. 2019. EMBO Mol Med. 11:e9266. PubMed
  19. Ding Y, et al. 2022. Cell Death Dis. 13:996. PubMed
  20. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  21. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  22. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  23. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  24. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  25. Sung PS, et al. 2022. JCI Insight. :. PubMed
  26. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  27. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  28. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
  29. Lee HN, et al. 2022. JCI Insight. 7:. PubMed
  30. Jones GS, et al. 2020. mSphere. 5:. PubMed
  31. Goldberg MF et al. 2018. Immunity. 49(6):1090-1102 . PubMed
  32. Kilgore AM, et al. 2020. J Immunol. 204:510. PubMed
RRID
AB_2562937 (BioLegend Cat. No. 127633)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

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