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首页 > 抗体蛋白 > 抗体
Biotin anti-mouse Ly-6G Antibody
产品名称：
Biotin anti-mouse Ly-6G Antibody
产品类别：
抗体
产品编号：
127603
产品应用：
127603
[价格]
规格 价格 库存
50µg ￥ 924 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo?studies or highly sensitive assays (Cat. No.?127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  21. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Barkaway A, et al. 2021. Immunity. . PubMed
  2. Haffner-Luntzer M, et al. 2017. Eur J Med Res. 10.1186/s40001-017-0264-y. PubMed
  3. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  4. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  5. Blazek K, et al. 2015. J Exp Med. 212:845. PubMed
  6. Haffner–Luntzer M, et al. 2019. Proc Natl Acad Sci U S A. 116:8615. PubMed
  7. Murata A, et al. 2020. Front Immunol. 11:775. PubMed
  8. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  9. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  10. Tang Y, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G156 - G165. PubMed
  11. ?lvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  12. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  13. Periasamy S, et al. 2016. PLoS Pathog. 12: 1005517. PubMed
  14. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  15. Lerman I, et al. 2019. Mol Cancer Res. 17:845. PubMed
  16. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  17. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  18. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  19. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  20. Vaibhav K, et al. 2018. J Exp Med. 215:2636. PubMed
  21. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  22. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  23. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  24. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  25. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  26. Moorlag SJCFM, et al. 2020. Cell Rep. 33:108387. PubMed
  27. Ryzhov S, et al. 2011. J Immunol. 187:6120. PubMed
  28. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  29. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  30. Muthukrishnan G, et al. 2021. Frontiers in Immunology. 12:651515. PubMed
  31. Popova T, et al. 2016. PLoS One. 11: 0163163. PubMed
  32. Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed
  33. Yang L, et al. 2012. J Immunol. 189:3741. PubMed
  34. Chen Z, et al. 2021. Front Immunol. 12:641874. PubMed
  35. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  36. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  37. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  38. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  39. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  40. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  41. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  42. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  43. Mohammadpour H, et al. 2021. Cell Rep. 37:109883. PubMed
  44. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  45. Sahakian E, et al. 2017. J Leukoc Biol. 102:475. PubMed
  46. Anderson DA, et al. 2022. J Exp Med. 219:. PubMed
  47. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  48. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  49. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  50. Huang X, et al. 2021. Immunity. . PubMed
  51. Sch?fer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  52. Morita Y, et al. 2022. Bone Res. 10:56. PubMed
  53. De Cock J, et al. 2016. Cancer Res . 76(23):6778-6784. PubMed
  54. Cai W, et al. 2019. J Neuroinflammation. 0.788194444. PubMed
  55. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  56. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
  57. Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed
  58. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_1186105 (BioLegend Cat. No. 127603) AB_1186108 (BioLegend Cat. No. 127604)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

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