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Alexa Fluor® 488 anti-mouse Ly-6G Antibody
产品名称：
Alexa Fluor® 488 anti-mouse Ly-6G Antibody
产品类别：
抗体
产品编号：
127625
产品应用：
127625
[价格]
规格 价格 库存
25µg ￥ 1488 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L?volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 μg/mL?is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for in vivo?studies or highly sensitive assays (Cat. No.?127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  21. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Barkaway A, et al. 2021. Immunity. . PubMed
  2. Sweet R, et al. 2017. J Immunol. 10.4049/jimmunol.1600861. PubMed
  3. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  4. Liu S, et al. 2020. Neuron. S0896-6273(20)30532-8.. PubMed
  5. Joy MT, et al. 2019. Cell. 176:1143. PubMed
  6. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  7. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  8. Wilson RP, et al. 2019. PLoS Pathog. 15:e1007745. PubMed
  9. Jahan S, et al. 2022. J Biol Chem. :102386. PubMed
  10. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  11. Stolp B, et al. 2022. Cell Rep. 38:110387. PubMed
  12. Craver BM, et al. 2020. Blood Adv. 4:312. PubMed
  13. Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed
  14. Ueki H, et al. 2020. Nat Protoc. 15:1041. PubMed
  15. Leung H, et al. 2022. Nat Commun. 13:5206. PubMed
  16. Green D, et al. 2020. Oncogene. 39:5553. PubMed
  17. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  18. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  19. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  20. Myerson JW, et al. 2021. Nat Nanotechnol. Online ahead of print. PubMed
  21. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  22. Pach E, et al. 2021. Cancers (Basel). 13:. PubMed
  23. Du J, et al. 2021. Mol Med Rep. 23:. PubMed
  24. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  25. Liu G, et al. 2022. Ann Transl Med. 10:693. PubMed
  26. Takizawa S, et al. 2022. Mol Med. 28:91. PubMed
  27. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
  28. Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed
  29. Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed
  30. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  31. Boras M, et al. 2017. J Exp Med. 214:851. PubMed
RRID
AB_2561339 (BioLegend Cat. No. 127625) AB_2561340 (BioLegend Cat. No. 127626)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

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