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PE anti-rat CD54 Antibody
产品名称:
PE anti-rat CD54 Antibody
产品类别:
抗体
产品编号:
202405
产品应用:
202405
[价格]
规格 价格 库存
100µg ¥ 2052 1

产品详情

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HEV-derived cell line Ax
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1,5 of acetone-fixed frozen sections and STUF-treated paraffin-embedded sections, immunoprecipitation2, in vitro and in vivo blocking of PMA-induced T cell blast aggregation and adhesion to HEV-derived endothelial cells and purified ICAM-1 protein2,3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Clark WM, et al. 1995. Mol. Chem. Neuropathol. 26:213. (IHC)
  2. Tamatani T, et al. 1990. Int. Immunol. 2:165. (IP)
  3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
  4. Willenborg DO, et al. 1996. J. Immunol. 157:1973.
  5. McKechnie NM, et al. 2002. IVOS 43:411. (IHC)
Product Citations
  1. Pele T, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_2121899 (BioLegend Cat. No. 202405)

Antigen Details

Structure
Ig superfamily, 85 kD
Distribution
High endothelial venules in lymphoid tissues, peripheral blood monocytes, dendritic cells, granulocytes, thymic stromal cells and weakly on T and B cells. Upregulated on activated cells and endothelial cells.
Function
Cellular adhesion, inflammation, immune reactions
Ligand/Receptor
CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, hyaluronan
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tamatani T, et al. 1990. Int. Immunol. 2:165.
2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
3. Turunen JP, et al. 1993. Scand. J. Immunol. 37:282.

Gene ID
15894 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org

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