Ultra-LEAF™ Purified anti-human/mouse TGF-&b-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2844	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human TGF-β1

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking activity of human TGF-β1 (500 pg/ml) in Mv1Lu-(caga)₁₂-Luc reporter cells. The ED₅₀ is 0.05 - 0.1 ?g/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: To measure TGF-?1, this antibody can be used as a detection antibody in sandwich ELISA format, and paired with the purified 21C11 antibody (Cat. No. 525301) as the capture antibody. Recombinant Human TGF-?1 (ELISA Std.) (Cat. No. 580709) can be used as the protein standard.

Blocking: Clone 19D8 LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is useful for in vitro blocking of human and mouse TGF-?1 bioactivity. The 19D8 clone does not inhibit the bioactivity of human TGF-?2 or TGF-?3 in the Mv1Lu-(caga)₁₂-Luc reporter cell assay.

Note: For testing TGF-?1 in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX? Kits (Cat. No. 436708 & 437708) are specially developed and recommended.

Product Citations

Li L, et al. 2021. Sci Adv. 7: . PubMed
Nakayama K, et al. 2020. Cell Immunol. 358:104237. PubMed
Yoon H, et al. 2021. Oncogenesis. 10:13. PubMed
Weinstock A, et al. 2021. Elife. :10. PubMed
Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
Shen Y, et al. 2022. Nat Commun. 13:3419. PubMed
Rivera CA, et al. 2022. Immunity. 55:129. PubMed

RRID

AB_2810653 (BioLegend Cat. No. 521707) AB_2810654 (BioLegend Cat. No. 521708)

Antigen Details

Structure: Homodimer
Distribution: TGF-β1 is secreted by numerous cells.
Function: TGF-β1 influences the fate of T cells into different subpopulations depending on the occurrence of other cytokines. TGF-β1 induces cell growth and survival, cell and tissue differentiation, development, inflammation, immunity, hematopoiesis, tissue remodeling, wound healing, and stimulates ECM deposition and angiogenesis. It is an essential mediator of pathologic scarring in fibrotic disorders.
Interaction: Multiple cells express the TGF-β1 receptors.
Ligand/Receptor: Heterodimeric receptor consisting of type I (TbRI) and type II (TbRII).
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Dubois CM, et al. 1995. J. Biol. Chem. 270:10618.
2. Dennler S, et al. 1998. EMBO J. 17:3091.
3. Annes JP, et al. 2003. J. Cell. Sci. 116:217.
4. Zou Z and Sun PD. 2004. Protein Expr. Purif. 37:265.
5. Park SH, 2005. J. Biochem. Mol. Biol. 38:9.
6. Valcourt U, et al. 2005. Mol. Biol. Cell. 16:1987.
7. Mucida D, et al. 2007. Science 317:256.
8. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 8:942.
9. Puthawala K, et al. 2008. Am. J. Respir. Crit. Care Med. 177:82.
10. Takatori H, et al. 2008. Mod. Rheumatol. 18:533.
11. Manel N, et al. 2008. Nat. Immunol. 9:641.
12. Veldhoen M, et al. 2008. Nat. Immunol. 9:1341.
13. Oida T, et al. 2011. PLoS ONE 6:e18365.
Gene ID: 7040 View all products for this Gene ID 21803 View all products for this Gene ID
UniProt: View information about TGF-beta1 on UniProt.org

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