Biotin anti-human/mouse TGF-β1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1344	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human TGF-β1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.0625 ?g/ml of the detection antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application Notes

ELISA Detection: To measure TGF-β1, this antibody can be used as a detection antibody in sandwich ELISA format, and paired with the purified 21C11 antibody (Cat. No. 525301) as the capture antibody. Recombinant Human TGF-β1 (ELISA Std.) (Cat. No. 580709) can be used as the protein standard.

Blocking: Clone 19D8 LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is useful for in vitro blocking of human and mouse TGF-β1 bioactivity. The 19D8 clone does not inhibit the bioactivity of human TGF-β2 or TGF-β3 in the Mv1Lu-(caga)₁₂-Luc reporter cell assay.

Note: For testing TGF-β1 in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX? Kits (Cat. No. 436708 & 437708) are specially developed and recommended.

Product Citations

Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed

RRID

AB_2562973 (BioLegend Cat. No. 521705)

Antigen Details

Structure: Homodimer
Distribution: TGF-β1 is secreted by numerous cells.
Function: TGF-β1 influences the fate of T cells into different subpopulations depending on the occurrence of other cytokines. TGF-β1 induces cell growth and survival, cell and tissue differentiation, development, inflammation, immunity, hematopoiesis, tissue remodeling, wound healing, and stimulates ECM deposition and angiogenesis. It is an essential mediator of pathologic scarring in fibrotic disorders.
Interaction: Multiple cells express the TGF-β1 receptors.
Ligand/Receptor: Heterodimeric receptor consisting of type I (TbRI) and type II (TbRII).
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Dubois CM, et al. 1995. J. Biol. Chem. 270:10618.
2. Dennler S, et al. 1998. EMBO J. 17:3091.
3. Annes JP, et al. 2003. J. Cell. Sci. 116:217.
4. Zou Z and Sun PD. 2004. Protein Expr. Purif. 37:265.
5. Park SH, 2005. J. Biochem. Mol. Biol. 38:9.
6. Valcourt U, et al. 2005. Mol. Biol. Cell. 16:1987.
7. Mucida D, et al. 2007. Science 317:256.
8. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 8:942.
9. Puthawala K, et al. 2008. Am. J. Respir. Crit. Care Med. 177:82.
10. Takatori H, et al. 2008. Mod. Rheumatol. 18:533.
11. Manel N, et al. 2008. Nat. Immunol. 9:641.
12. Veldhoen M, et al. 2008. Nat. Immunol. 9:1341.
13. Oida T, et al. 2011. PLoS ONE 6:e18365.
Gene ID: 7040 View all products for this Gene ID 21803 View all products for this Gene ID
UniProt: View information about TGF-beta1 on UniProt.org

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