Purified anti-TPX2 Antibody
- 产品名称：
- Purified anti-TPX2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 628002
- 产品应用：
- 628002
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Amino Acid: 1-220 of human TPX2
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Fung TK, et al. 2007. Mol Biol Cell. 18:1861. PubMed
- Warner SL, et al. 2009. Clin Cancer Res. 15:6519. PubMed
- Bibby RA, et al. 2009. J. Biol Chem. PubMed
- Rohena CC, et al. 2013. Biochem Pharmacol. 85:1104. PubMed.
- Product Citations
-
- Bibby R, et al. 2009. J Biol Chem. 284:33177. PubMed
- Warner S, et al. 2009. Clin Cancer Res. 5.152083333. PubMed
- Fung T, et al. 2007. Mol Biol Cell . 2.042361111. PubMed
- Eot-Houllier G, et al. 2010. J Biol Chem. 285:29556. PubMed
- Tagal V, et al. 2017. Nat Commun. 8:14098. PubMed
- RRID
- AB_2287863 (BioLegend Cat. No. 628002)
- Structure
- Nuclear protein, contains two coiled-coil domains, approximately 100 kD
- Distribution
-
Nuclear, during mitosis strictly associated with spindle pole and mitotic spindle. In G2/S cell cycle, diffusely distributed throughout nucleus. Highly expressed in lung carcinomas cell lines, but not in normal lung tissues.
- Function
- This protein is exclusively expressed in proliferating cells from the G1/S transition to the end of cytokinesis. Thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays. May also be involved in targeting Aurora-A kinase to spindle.
- Interaction
- Interacts with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1,α-tubulin, and nucleolin among others
- Modification
- Phosphorylation (S738)
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
-
1. Gruss OJ, et al. 2002. Nature Cell Biol. 4:871.
2. Heidebrecht HJ, et al. 1997. Blood 90:226.
3. Kufer TA, et al. 2002. J. Cell Biol. 158:617.
- Gene ID
- 22974 View all products for this Gene ID
- UniProt
- View information about TPX2 on UniProt.org
