Anti-Alpha-I Spectrin Antibody (Previously Covanc-上海雅吉生物科技有限公司

规格	价格	库存
200µL	￥ 6972	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant α spectrin SH3 domain

Formulation

Phosphate-buffered solution (no preservatives or carrier proteins).

Preparation

The antibody was purified by affinity chromatography.

Concentration

1 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.

Application

WB -?Quality tested
ICC -?Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 ?g per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in Western Blotting (WB). Additional reported applications include immunocytochemistry^{1, 2}.

Clone 17C7 is a specific marker for human, rat and mouse alpha I spectrin. It reacts with erythroid spectrin in red blood cells and in cerebellum. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In cerebellar cell cultures, clone 17C7 labels cell bodies consistent with localization of erythroid spectrin. In NIH 3T3 cells, clone 17C7 reacts with a 280 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes^{1, 2}.

This antibody was developed against a recombinant alpha I spectrin (erythroid spectrin) SH3 domain.

Application References (PubMed link indicates BioLegend citation)