Ultra-LEAF™ Purified anti-mouse CD137 Antibo
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD137 Antibo
- 产品类别：
- 抗体
- 产品编号：
- 106113
- 产品应用：
- 106113
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of 4-1BB-mediated T cell proliferation2. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 106108).
- Application References
(PubMed link indicates BioLegend citation) -
- Lee SW, et al. 2005. J. Immunol. 174:6803. (FC)
- Zheng G, et al. 2004. J. Immunol. 173:2428. (Block)
- Zheng H and G. G. Meadows 2005. J. Leukocyte Biol. 78:1070. (FC) PubMed
- Nishimoto H, et al. 2005. Blood 106:4241. (FC)
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- RRID
- AB_2832348 (BioLegend Cat. No. 106113) AB_2832349 (BioLegend Cat. No. 106114) AB_2832350 (BioLegend Cat. No. 106115) AB_2832351 (BioLegend Cat. No. 106116) AB_2832352 (BioLegend Cat. No. 106117) AB_2876416 (BioLegend Cat. No. 106118)
- Structure
- TNF-R superfamily, 39 kD
- Distribution
-
Activated T cells and B cells
- Function
- T-B cell costimulation
- Ligand/Receptor
- 4-1BB ligand (CDw137L)
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Alderson MR, et al. 1994. Eur. J. Immunol. 24:2219.
3. Schwarz H, et al. 1996. Blood 87:2839.
- Gene ID
- 21942 View all products for this Gene ID
- UniProt
- View information about CD137 on UniProt.org
