Biotin anti-mouse CD137 Antibody
- 产品名称:
- Biotin anti-mouse CD137 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 106103
- 产品应用:
- 106103
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of 4-1BB-mediated T cell proliferation2. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 106108).
- Application References
(PubMed link indicates BioLegend citation) -
- Lee SW, et al. 2005. J. Immunol. 174:6803. (FC)
- Zheng G, et al. 2004. J. Immunol. 173:2428. (Block)
- Zheng H and G. G. Meadows 2005. J. Leukocyte Biol. 78:1070. (FC) PubMed
- Nishimoto H, et al. 2005. Blood 106:4241. (FC)
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Product Citations
-
- Wan D, et al. 2021. Exp Mol Med. 53:654. PubMed
- Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
- Itoh A, et al. 2019. Front Immunol. 2.198611111. PubMed
- Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
- RRID
- AB_313240 (BioLegend Cat. No. 106103) AB_313241 (BioLegend Cat. No. 106104)
- Disclaimer
This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
- Structure
- TNF-R superfamily, 39 kD
- Distribution
-
Activated T cells and B cells
- Function
- T-B cell costimulation
- Ligand/Receptor
- 4-1BB ligand (CDw137L)
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Alderson MR, et al. 1994. Eur. J. Immunol. 24:2219.
3. Schwarz H, et al. 1996. Blood 87:2839.
- Gene ID
- 21942 View all products for this Gene ID
- UniProt
- View information about CD137 on UniProt.org