Ultra-LEAF™ Purified anti-mouse CD3 Antibody
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 100239
- 产品应用：
- 100239
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- γδTCR-positive T-T hybridoma D1
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
IHC-F, IP, ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
- Application References
(PubMed link indicates BioLegend citation) -
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
- Gkountidi AO, et al. 2021. Cancer Immunol Res. 9:748. PubMed
- Saito S, et al. 2020. Nutrients. 12:. PubMed
- Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
- Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
- Fajstova A, et al. 2020. Cells. 9:00. PubMed
- Watson MJ, et al. 2021. Nature. 591:645. PubMed
- Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
- Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
- De Cicco P, et al. 2019. British Journal of Pharmacology. 177(4):884-897. PubMed
- Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
- Xu L, et al. 2022. Nat Commun. 13:6881. PubMed
- Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
- Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
- RRID
- AB_2810313 (BioLegend Cat. No. 100239) AB_2561487 (BioLegend Cat. No. 100238) AB_2810314 (BioLegend Cat. No. 100253) AB_2810315 (BioLegend Cat. No. 100254) AB_2810316 (BioLegend Cat. No. 100255) AB_2810317 (BioLegend Cat. No. 100256)
- Structure
- Ig superfamily, CD3/TCR, 20 kD
- Distribution
-
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- Antigen recognition, TCR signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen/MHC-complex
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
- Gene ID
- 12502 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
