Spark NIR™ 685 anti-mouse CD3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1692	1

规格

价格

库存

25µg

￥ 1692

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

γδTCR-positive T-T hybridoma D1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark NIR? 685 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Spark NIR? 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.

Application References (PubMed link indicates BioLegend citation)

Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

RRID

AB_2832259 (BioLegend Cat. No. 100261) AB_2832260 (BioLegend Cat. No. 100262)

Antigen Details

Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

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