PE/Dazzle™ 594 anti-mouse CD3 Antibody
- 产品名称：
- PE/Dazzle™ 594 anti-mouse CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 100245
- 产品应用：
- 100245
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- γδTCR-positive T-T hybridoma D1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
- Application References
(PubMed link indicates BioLegend citation) -
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
- Ma F, et al. 2020. Cell Death Dis. 1.172222222. PubMed
- Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
- Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
- Guo HF, et al. 2021. Commun Biol. 4:482. PubMed
- Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
- He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
- Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
- Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
- Lu S, et al. 2020. Arterioscler Thromb Vasc Biol. 40:394. PubMed
- Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
- Tan X, et al. 2021. Sci Adv. 7: . PubMed
- Padhye A, et al. 2019. Sci Rep. 9:4819. PubMed
- RRID
- AB_2565882 (BioLegend Cat. No. 100245) AB_2565883 (BioLegend Cat. No. 100246)
- Structure
- Ig superfamily, CD3/TCR, 20 kD
- Distribution
-
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- Antigen recognition, TCR signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen/MHC-complex
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
- Gene ID
- 12502 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
