Biotin anti-mouse CD3 Antibody
- 产品名称：
- Biotin anti-mouse CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 100244
- 产品应用：
- 100244
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- γδTCR-positive T-T hybridoma D1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
IHC-F -?Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. For immunohistochemistry, a concentration range of 5.0?- 10??g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
- Application References
(PubMed link indicates BioLegend citation) -
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
- Thierry GR, et al. 2018. J Exp Med. 215:2972. PubMed
- Shao B, et al. 2018. Mol Med Rep. 18:920. PubMed
- Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
- Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
- Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
- Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
- Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
- Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
- Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
- Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
- Yu VWC, et al. 2017. Cell. 168:944. PubMed
- Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
- Wang Y, et al. 2021. Signal Transduct Target Ther. 6:409. PubMed
- Shen Z, et al. 2021. iScience. 24:103014. PubMed
- Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
- Dong S, et al. 2021. Nature. 591:117. PubMed
- Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
- Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
- Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
- Bost P, et al. 2020. Cell. 181(7):1475-1488. PubMed
- Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
- Zeis P, et al. 2020. Immunity. 53:775. PubMed
- Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
- Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
- Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
- Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed
- S?wen P et al. 2018. eLife. 7 pii: e41258. PubMed
- Lee A, et al. 2022. Nat Commun. 13:549. PubMed
- RRID
- AB_2563946 (BioLegend Cat. No. 100243) AB_2563947 (BioLegend Cat. No. 100244)
- Structure
- Ig superfamily, CD3/TCR, 20 kD
- Distribution
-
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- Antigen recognition, TCR signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen/MHC-complex
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
- Gene ID
- 12502 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
