Alexa Fluor® 647 anti-mouse CD3 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 100209
- 产品应用：
- 100209
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- γδTCR-positive T-T hybridoma D1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F, 3D IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L?volume.?For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 ?g/mL?is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
- Application References
(PubMed link indicates BioLegend citation) -
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Sun L, et al. 2021. Cancer Cell. :. PubMed
- Hiraoka N, et al. 2011. Gastroenterology. 140:310. PubMed
- Lin YN, et al. 2022. Oncoimmunology. 11:2027136. PubMed
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- Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
- Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
- Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
- Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
- Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed
- Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
- Zeng Z, et al. 2022. Oncogene. :. PubMed
- Rassy D, et al. 2020. J Neuropathol Exp Neurol. 226:79. PubMed
- Xiong S 2013. J Immunol. 190:3267. PubMed
- Sluis T, et al. 2015. Clin Cancer Res. 21:781. PubMed
- Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
- Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
- Cecchinato V, et al. 2017. J Immunol. 198(1):184-195. PubMed
- Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
- Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
- Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed
- Ardehali R, et al. 2013. Proc Natl Acad Sci U S A. 110:3405. PubMed
- Agudo J et al. 2018. Immunity. 48(2):271-285 . PubMed
- Chen S, et al. 2015. Blood. 126: 103 - 112. PubMed
- Crinier A, et al. 2018. Immunity. 49:971. PubMed
- Bárcena C et al. 2019. EBioMedicine. 43:513-524 . PubMed
- Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
- Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
- Chang H, et al. 2016. J Immunol. 197: 2473 - 2484. PubMed
- Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
- O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
- RRID
- AB_389323 (BioLegend Cat. No. 100209)
- Structure
- Ig superfamily, CD3/TCR, 20 kD
- Distribution
-
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- Antigen recognition, TCR signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen/MHC-complex
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
- Gene ID
- 12502 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
