Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0?- 5.0??g/mL?is suggested.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L?volume.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

1. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
2. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

1. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
2. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
3. Matsumoto R, et al. 2021. Front Pharmacol. 12:715752. PubMed
4. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
5. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
6. Liu M, et al. 2020. Nature. 587:115. PubMed
7. Wong HS, et al. 2021. Cell. . PubMed
8. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
9. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
10. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
11. Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed
12. Chung YM, et al. 2021. J Immunother Cancer. 9:. PubMed
13. Palacio L, et al. 2019. Aging Cell. 18(4):e12971. PubMed
14. Matryba P, et al. 2020. J Immunol. 1395:204. PubMed
15. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
16. Pantelidou C, et al. 2022. NPJ Breast Cancer. 8:102. PubMed
17. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
18. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed

Thymocytes (differentiation dependent), mature T cells, NK-T cells

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.