Alexa Fluor? 647 anti-ATG5 Antibody
- 产品名称：
- Alexa Fluor? 647 anti-ATG5 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 847409
- 产品应用：
- 847409
- Verified Reactivity
- Human, Mouse, Rat, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- ATG5 peptide conjugated to KLH.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Reactivity to Drosophila was only verified with the purified format.
- Product Citations
-
- Mece O, et al. 2022. Nat Commun. 13:2760. PubMed
- RRID
- AB_2814562 (BioLegend Cat. No. 847409) AB_2814563 (BioLegend Cat. No. 847410)
- Structure
- Human ATG5 is a 275 amino acid protein with a molecular mass of 32 kD.
- Distribution
-
Tissue distribution: Ubiquitous.
Cellular distribution: Vacuole, lysosome, and to a lesser extent mitochondria.
- Function
- ATG5 is involved in many cellular processes including autophagic vesicle formation, mitochondrial quality control after oxidative damage, innate anti-viral immune response, and apoptosis.
- Interaction
- ATG12, LC3B, ATG16, TECPR1, Autophagy-linked FYVE protein.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
- Molecular Family
- Autophagosome Markers
- Antigen References
-
1. Uchiyama Y, et al. 2008. Histochem. Cell Biol. 129:407. PubMed
2. Wu H, et al. 2014. Int. J. Biol. Sci. 10:1072. PubMed
3. Subramani S, Malhotra V. 2013. EMBO Rep. 14(2):143. PubMed
- Gene ID
- 9474 View all products for this Gene ID
- UniProt
- View information about ATG5 on UniProt.org
