Biotin anti-human CD140a (PDGFRα) Antibody
- 产品名称：
- Biotin anti-human CD140a (PDGFRα) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 323503
- 产品应用：
- 323503
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NIH 3T3 cells transfected with human PDGFRalpha
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Miyazaki S et al. In:Leukocyte Typing VI Kishimoto et al. Eds, Garland Publishing Inc, New York 1998 pp 3-20.
- Lottaz C, et al. 2010. Cancer Res. 70:2030. PubMed
- Ricono JM, et al. 2009. Am. J. Physiol. Renal Physiol. 296:F406. (IF)
- Guarnerio J, et al. 2015. Cancer Discov. 5:396. PubMed
- Product Citations
-
- Niwa A, et al. 2011. PLoS One. 6:e22261. PubMed
- Evseenko D, et al. 2010. Proc Natl Acad Sci U S A. 107:13742. PubMed
- Ishimine H, et al. 2013. Biochem Biophys Res Commun. 438:753. PubMed
- RRID
- AB_755997 (BioLegend Cat. No. 323503) AB_2162487 (BioLegend Cat. No. 323504)
- Structure
- Cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family.
- Distribution
-
Widely expressed on a variety of mesenchymal-derived cells.
- Function
- Stimulation of cell proliferation; mitogenic activity for cells of mesenchymal origin. Knock-out studies have implicated an essential role for CD140a in kidney development. Has been implicated in basal cell carcinoma and gastric stromal cell tumors.
- Interaction
- Interacts with Crk, as well as a variety of adaptor molecules and signaling intermediates (Grb2, Grb14, SHP2, others). Has also been shown to associate with integrin β3, caveolin-1, and nexin sorting molecules
- Ligand/Receptor
- Binds to A-chain containing PDGF molecules and protease-activated PDGF-C molecules
- Modification
- Multiple tyrosine phosphorylation sites (Y720, Y731, Y742, Y754, Y762, Y767, Y768, Y988, Y993, Y1018)
- Cell Type
- Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Gronwald RG, et al. 1988. Proc. Natl. Acad. Sci. USA 85:3435.
2. Gilbertson DG, et al. 2001. J. Biol. Chem. 276:27406.
3. Seifert RA, et al. 1989. J. Biol. Chem. 264:8771.
4. Rupp E, et al. 1994. Eur. J. Biochem. 225:29.
- Gene ID
- 5156 View all products for this Gene ID
- UniProt
- View information about CD140a on UniProt.org
