Alexa Fluor® 488 anti-human CD206 (MMR) Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1488	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Purified human mannose receptor

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting¹, blocking of ligand binding^1,2, immunofluorescence³, and immunohistochemical staining of acetone-fixed frozen tissue sections¹. The Utra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 321149 and 321150).

Application References (PubMed link indicates BioLegend citation)

Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block)
Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block)
Kato M, et al. 2007. J. Immunol. 179:6052. (IF)

Product Citations

Gattas MJ, et al. 2021. International Journal of Molecular Sciences. 22(10):. PubMed
Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
Borowicz S, et al. 2021. PLOS ONE. 16(6):e0252197. PubMed
Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed
Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed

RRID

AB_571874 (BioLegend Cat. No. 321113) AB_571875 (BioLegend Cat. No. 321114)

Antigen Details

Structure: Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution: Macrophages, dendritic cells, hepatic and lymphatic endothelial cells
Function: Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor: Mannose, fucose, N-acetyl glucosamine
Cell Type: Dendritic cells, Endothelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303
2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469.
4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934.
5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.
Gene ID: 4360 View all products for this Gene ID
UniProt: View information about CD206 on UniProt.org

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