Purified anti-CD209/CD299 (DC-SIGN/L-SIGN) Antibod
- 产品名称：
- Purified anti-CD209/CD299 (DC-SIGN/L-SIGN) Antibod
- 产品类别：
- 抗体
- 产品编号：
- 845002
- 产品应用：
- 845002
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- DC-SIGN DNA.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone 14E3G7 has been shown to recognize both CD209 and CD299 transfectants.1
- Application References
(PubMed link indicates BioLegend citation) -
- Granelli-Piperno A, et al. 2005. J. Immunol. 175:4265.
- Product Citations
-
- Lempp FA, et al. 2021. Nature. 598:342. PubMed
- RRID
- AB_2566004 (BioLegend Cat. No. 845002)
- Structure
- DC-SIGN is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. DC-SIGNR (DC-SIGN-related molecule) is a type II integral membrane protein that is 77% identical to DC-SIGN.
- Distribution
-
CD209 is expressed on myeloid dendritic cells, placental macrophages, and liver and placental endothelial cells. CD299 is expressed on liver sinusoidal endothelial cells, lung and placental endothelial cells, type 2 alveolar cells, and endothelial cells of lymph nodes.
- Function
- CD299 mediates interactions between dendritic cells and resting T cells and has a high affinity for HIV-1 gp120. CD209 plays a critical role in capturing and internalizing pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates the transport of HIV to the proteasome.
- Interaction
- CD209 is a receptor of viruses such as HIV-1, West Nile Virus, hepatitis C virus, some bacteria, and parasites. CD299 binds carbohydrate structures of pathogens such as hepatitis C virus, Mycobacterium, etc.
- Ligand/Receptor
- CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTNA2A1). CD299 is a receptor for ICAM-3 (CD50).
- Cell Type
- Dendritic cells, Endothelial cells, Macrophages
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fauci A. 1996. Nature 384:529.
2. Yokoyama-Kobayashi M, et al. 1999. Gene 228:161.
3. Soilleux EJ, et al. 2000. J. Immunol. 165:2937.
4. Pohlmann S, et al. 2001. Proc. Natl. Acad. Sci. USA 98:2670.
5. Bashirova AA, et al. 2001. J. Exp. Med. 193:671.
6. Lai WK, et al. 2006. Am. J. Pathol. 169:200.
- Gene ID
- 30835 View all products for this Gene ID 10332 View all products for this Gene ID
- UniProt
- View information about CD209 on UniProt.org
