Purified anti-XBP-1s Antibody
- 产品名称：
- Purified anti-XBP-1s Antibody
- 产品类别：
- 抗体
- 产品编号：
- 647502
- 产品应用：
- 647502
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- XBP-1s recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ChIP -?Verified
ICC, IHC-P - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 to 2.0 ?g per mL.?For ChIP application, use 2 ?g of antibody per 10 ?g of chromatin per IP. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence14 and immunohistochemical staining of PFA-fixed paraffin-embedded sections1,14.
- Additional Product Notes
Using this antibody at a ratio higher than 1:5 (?g antibody per ?g of chromatin) will increase background.
- Application References
(PubMed link indicates BioLegend citation) -
- Tsang KY, et al. 2007. PLOS Biol. 5:568. (IHC) PubMed
- Farhan H, et al. 2008. EMBO J. 27:2043 (WB) PubMed
- Farhan H, et al. 2010. J. Cell Biol. 189:997. PubMed
- Feng-Jin G, et al. 2010. Cell Signal. [Epub ahead of print]
- Wang L, et al. 2011. Hum Mol Genet. PubMed
- Li J, et al. 2011. J. Biol Chem. 286:4912. PubMed
- Wang L, et al. 2011. Hum Mol Genet. 20:1008. PubMed
- Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed
- Shirley CM, et al. 2011 Blood. 117:6297. PubMed
- Vidal RL., et al. 2012. Hum Mol Genet. PubMed
- Byrd AE, et al. 2012. J Cell Biol. 196:689. PubMed
- Dickie LJ, et al. 2012. Ann rheum Dis. 71:2035. PubMed
- Bonetti P, et al. 2013. Blood. 122:2233. PubMed
- Maestre L, et al. 2009. Haematologica. 94:419. (IF, IHC)
- Rodriguez M, et al. 2014. J Biol Chem. 289:22942. PubMed
- Product Citations
-
- Hattori K, et al. 2021. Oncol Lett. 22:680. PubMed
- Zielke S, et al. 2020. Autophagy. 1:. PubMed
- Li J et al. 2018. Cell chemical biology. 25(11):1350-1358 . PubMed
- Dickie L, et al. 2012. Ann Rheum Dis. 71:2035. PubMed
- Wang L, et al. 2011. Hum Mol Genet. 1.533333333. PubMed
- Syx D, et al. 2021. PLoS Genet. 17:e1009339. PubMed
- Zang M, et al. 2020. Oncogenesis. 9:31. PubMed
- Cai X, et al. 2015. Exp Cell Res. Available online 29 August 2015. PubMed
- Byrd A, et al. 2012. J Cell Biol. 196:689. PubMed
- Miyoshi A, et al. 2019. FASEB J. 33:3575. PubMed
- Li X, et al. 2018. Sci Rep. 7:2013. PubMed
- Lindner P, et al. 2020. Cell Commun Signal. 18:12. PubMed
- Shirley C, et al. 2011. Blood. 117:6297. PubMed
- Rinaldi R, et al. 2021. Cells. 10:. PubMed
- Vidal R, et al. 2012. Hum Mol Genet. 2.434027778. PubMed
- Luhr M, et al. 2019. J Biol Chem. 294:8197. PubMed
- Farhan H, et al. 2010. J Cell Biol. 189:997. PubMed
- Li J, et al. 2011. J Biol Chem. 286:4912. PubMed
- Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed
- Talty A, et al. 2019. Cell Death Dis. 10:622. PubMed
- Walter F, et al. 2022. Front Cell Dev Biol. 10:915065. PubMed
- Chen W, et al. 2022. Nat Commun. 13:6796. PubMed
- Efimova I, et al. 2021. Front Immunol. 12:643144. PubMed
- Devireddy S, et al. 2022. J Cell Biol. 221:. PubMed
- RRID
- AB_2241744 (BioLegend Cat. No. 647501) AB_2241743 (BioLegend Cat. No. 647502)
- Distribution
-
Ubiquitously expressed.
- Function
- Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
- Biology Area
- Cell Biology
- Antigen References
-
1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem.268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154.
- Gene ID
- 7494 View all products for this Gene ID
- UniProt
- View information about XBP-1s on UniProt.org
