Purified anti-CD171 (L1) Antibody (Previously Cov
- 产品名称：
- Purified anti-CD171 (L1) Antibody (Previously Cov
- 产品类别：
- 抗体
- 产品编号：
- 826701
- 产品应用：
- 826701
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Clone 14.10 was raised to the ectodomain (amino acids M1 to E1123) of human L1 by using SKOV3ip cells as immunogen.
- Formulation
- Phosphate-buffered solution with BSA + 0.1% NaN3.
- Preparation
- The antibody was purified by affinity chromatography.
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
? IHC: ≥1:40 for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: Not required
Incubation: 20 minutes at room temperature
- Application Notes
This antibody is effective in immunohistochemistry (IHC).
By Western blot analysis, the L1-14.10 epitope was mapped to the third Ig domain. Clone 14.10 recognizes L1, a 200-220 kD transmembrane glycoprotein that functions as an adhesion molecule.
- Application References
(PubMed link indicates BioLegend citation) -
- Wolterink, et al. Therapeutic Antibodies to Human L1CAM: Functional Characterization and Application in a Mouse Model for Ovarian Carcinoma. Cancer Res.; 70: 2504-2515, Mar 2010.
- Huszar M, et al. Expression profile analysis in multiple human tumors identifies L1 (CD17as a molecular marker for differential diagnosis and targeted therapy. Human Pathology , Vol. 37, Issue 8, p1000-1008, Jun 2006. (First publication listed, but needs to be updated.)
- Huszar M, Moldenhauer G, Gschwend V, Ben-Arie A, Altevogt P, Fogela, M. Expression profile analysis in multiple human tumors Identifies L1 (CD171)as a molecular marker for differential diagnosis and targeted therapy. Hum Pathol **Article in Press** accepted 24 March, 2006
- Kaifi JT, Heidtmann S, et al. Absence of L1 in pancreatic masses distinguishes adenocarcinomas from poorly differentiated neuroendocrine carcinomas. Anticancer Res 26(2A):1167-70, 2006.
- Kaifi JT, et al. L1(CD17is highly expressed in gastrointestinal stromal tumors. Mod Pathol 19(3):399-406, 2006.
- Meier F, et al. The adhesion molecule L1 (CD17promotes melanoma progression. Int J Cancer 119(3):549-55, 2006.
- Stoeck A, et al. A role for exosomes in the constitutive and stimulus-induced ectodomain cleavage of L1 and CD44. Biochem J 393:609-18, 2006.
- Gutwein P, et al. Cleavage of L1 in exosomes and apoptotic membrane vesicles released from ovarian carcinoma cells.
- Clin Cancer Res 11(7):2492-501, 2005.
- Product Citations
-
- Oswald J, et al. 2021. Mol Ther Methods Clin Dev. 21:180. PubMed
- Ng PK, et al. 2018. Cancer Cell. 33:450. PubMed
- Weinberger V, et al. 2019. Front Oncol. 9:265. PubMed
- Karnezis AN, et al. 2017. J Pathol Clin Res. 3:279. PubMed
- Er EE, et al. 2018. Nat Cell Biol. 1.504166667. PubMed
- RRID
- AB_2564904 (BioLegend Cat. No. 826701)
