Ultra-LEAF™ Purified anti-human IL-16 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
1mg	￥ 9180	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Peptide corresponding to amino acids 615 to 630 (RRKSLQSKETTAAGDS) of IL-16

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA², inhibition of T cell chemotaxis² and immunohistochemical staining of frozen lymph node sections³.

Application References (PubMed link indicates BioLegend citation)

Cruikshank WW, et al. 1994. P. Natl Acad Sci USA 91:5109. (ICFC)
Keane J, et al. 1998. J. Immunol. 160:5945. (Block, ELISA)
Chupp GL, et al. 1998. J. Immunol. 161:3114. (Block, IHC)
Stoitzner P, et al. 2001. J. Invest. Dermatol. 116:641. (ICFC)
Ren F, et al. 2005. J. Immunol. 174:2738. (ICFC)

RRID

AB_2563454 (BioLegend Cat. No. 519108)

Antigen Details

Structure: 12 kD protein with a PDZ domain, product of the cleavage of pro-IL-16 by caspase-3, forms tetramers.
Distribution: Lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts.
Function: Chemoattractant for CD4⁺ T cells, monocytes, and eosinophils. Upregulates CD25 and class II expression on T cells. Inhibits HIV-1 replication.
Ligand/Receptor: CD4.
Cell Type: Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Mast cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Bellon M, et al. 2010. Blood 115:2441.
2. Rofani C, et al. 2009. Stem Cells Dev. 18:671.
3. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467.
4. Gu Y, et al. 2008. J. Immunol. 181:4418.
5. McFadden C, et al. 2007. J. Immunol. 179:6439.
6. Qi JC, et al. 2006. Blood 107:135.
Gene ID: 3603 View all products for this Gene ID
UniProt: View information about IL-16 on UniProt.org

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