Alexa Fluor® 647 anti-mouse I-E-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C3H mouse skin graft and splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity¹, immunoprecipitation², in vitro induction of B-cell differentiation³, in vitro blocking of antigen presentation⁴, and immunohistochemical staining^5,6 of acetone-fixed frozen sections.

Application References (PubMed link indicates BioLegend citation)

Ozato K, et al. 1980. J. Immunol. 124:533.
Nei U, et al. 1994. Int. Immunol. 6:61. (IP)
Harton JA, et al. 1991. Immunogenetics 34:358.
Roy M, et al. 1995. Eur. J. Immunol. 25:596.
Farr A, et al. 1996. Eur. J. Immunol. 26:3185. (IHC)
Desmet C, et al. 2004. J. Immunol. 173:5766. (IHC)
Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)

Product Citations

Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed

RRID

AB_493214 (BioLegend Cat. No. 110211)

Antigen Details

Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2^b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14969 View all products for this Gene ID
UniProt: View information about I-Ek on UniProt.org

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