Purified anti-CD325 (N-Cadherin) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 648	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human N-cadherin cytoplasmic domain fused to recombinant MBP.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB - Quality tested
ICC, IHC-P, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunfluorescence microscopy^1,2,3,6, immunohistochemical staining⁴, and immunoprecipitation^2,5,7.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 844703) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Wahl JK, et al. 2003. J. Biol. Chem. 278:17269. (WB, IF)
Knudsen KA, et al. 1995. J. Cell Biol. 130:67. (WB, IF, IP)
Machell NH, et al. 2000. Biol. Reprod. 63:797. (WB, IF)
Han AC, et al. 1997. Hum. Pathol. 28:641. (IHC)
Van Aken EH, et al. 2002. Invest Ophthalmol. Vis. Sci. 43:595. (WB, IP)
Tian G, et al. 2009. J. Biol. Chem. 284:18980. (IF)
Nieman MT. 1999. J. Cell Biol. 147:631. (IP, WB)

RRID

AB_2750044 (BioLegend Cat. No. 844701) AB_2565806 (BioLegend Cat. No. 844702)

Antigen Details

Structure: Member of cadherin family, single pass transmembrane protein, extracellular region consists of five extracellular cadherin domains, one cytoplasmic domain, 130 kD.
Distribution: Cell membrane, stem cells, myeloblasts, endothelial cells, fibroblasts, neural and muscle tissues, and some types of carcinoma cells.
Function: Embryonic development, maintain tissue architecture, cell sorting and cell adhesion in tissues, promote motility, cell junction organization, pre and post synaptic adhesion, and ERK signaling.
Interaction: Catenins.
Cell Type: Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Hirano S, et al. 2012. Physiol. Rev. 92:597.
Gene ID: 1000 View all products for this Gene ID
UniProt: View information about CD325 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们