Ultra-LEAF™ Purified anti-Syntrophin Antibod
- 产品名称：
- Ultra-LEAF™ Purified anti-Syntrophin Antibod
- 产品类别：
- 抗体
- 产品编号：
- 845103
- 产品应用：
- 845103
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The epitope has been mapped to amino acids 108-120 within the a-syntrophin PDZ domain.
- Application References
(PubMed link indicates BioLegend citation) -
- Froehner S, et al. 1987. J. Cell Biol. 104:1633. (WB, IHC)
- Gee S, et al. 1998. J. Neurosci. 18:128. (IP)
- Ort T, et al. 2001. EMBO 20:4013. (IP, WB)
- Adams M, et al. 2001. J. Cell Biol. 155:113. (IF)
- Williams M, et al. 1999. J. Cell Biol. 144:1259. (IHC)
- RRID
- AB_2566134 (BioLegend Cat. No. 845103)
- Structure
- Monomer and homodimer. Syntrophins consist of a conserved domain, two pleckstin homology domains, a PDZ domain, and a highly conserved C-terminal syntrophin unique (SU) domain. Expected MW: 58 kD.
- Function
- Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex.
- Interaction
- Interacts with the other members of the syntrophin family SNTB1 and SNTB2; SGCG and SGCA of the dystrophin glycoprotein complex; NOS1; GRB2; the sodium channel proteins SCN4A and SCN5A; F-actin and calmodulin; Interacts with MYOC.
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 6640 View all products for this Gene ID
- UniProt
- View information about Syntrophin on UniProt.org
