Purified anti-human CD184 (CXCR4) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 996	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Chimpanzee, Sooty Mangabey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

CP-MAC-infected Sup-T1 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
ICC, CyTOF? - Verified
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 10⁶ cells in 100 ?L?volume or 100 ?L?of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections¹¹, immunocytochemistry³, immunofluorescence microscopy^2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-1^4,5. Clone 12G5 may not be suitable for Western blotting.¹⁰ The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).

Application References (PubMed link indicates BioLegend citation)

McKnight A, et al. 1997. J. Virol. 71:1692.
Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
Ullrich CK, et al. 2000. Blood 96:1438. (Block)
Murga M, et al. 2005. Blood 105:1992. (IF)
Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
Isnardi I, et al. 2010. Blood 115:5026. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Fischer T, et al. 2008. PLoS One 3:e4069.
Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)

Product Citations

Mousset CM, et al. 2018. Oncoimmunology. 7:e1488565. PubMed
Thompson B, et al. 2007. J Biol Chem. 282:9547. PubMed
Ga?án-Gómez I, et al. 2022. Nat Med. . PubMed
Kickler K, et al. 2012. J Immunol. 188:5303. PubMed
Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
Kondo N, et al. 2020. Cancers (Basel). 12:00. PubMed
Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed

RRID

AB_314607 (BioLegend Cat. No. 306501) AB_314608 (BioLegend Cat. No. 306502)

Antigen Details

Structure: Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD
Distribution: T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells
Function: B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
Ligand/Receptor: SDF-1 receptor, coreceptor for X4 HIV-1
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.
Gene ID: 7852 View all products for this Gene ID
UniProt: View information about CD184 on UniProt.org

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