PE/Cyanine5 anti-human CD184 (CXCR4) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1800	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Chimpanzee, Sooty Mangabey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

CP-MAC-infected Sup-T1 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections¹¹, immunocytochemistry³, immunofluorescence microscopy^2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-1^4,5. Clone 12G5 may not be suitable for Western blotting.¹⁰ The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

McKnight A, et al. 1997. J. Virol. 71:1692.
Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
Ullrich CK, et al. 2000. Blood 96:1438. (Block)
Murga M, et al. 2005. Blood 105:1992. (IF)
Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
Isnardi I, et al. 2010. Blood 115:5026. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Fischer T, et al. 2008. PLoS One 3:e4069.
Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)

Product Citations

Herati R, et al. 2014. J Immunol. 193:3528. PubMed
Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
Mold JE, et al. 2021. Cell Reports. 35(8):109174. PubMed
Sciaranghella G, et al. 2015. J Infect Dis. 212: 1397 - 1401. PubMed
Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed
Wilson TL, et al. 2022. Cancer Discov. 12:2098. PubMed

RRID

AB_314613 (BioLegend Cat. No. 306507) AB_314614 (BioLegend Cat. No. 306508)

Antigen Details

Structure: Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD
Distribution: T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells
Function: B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
Ligand/Receptor: SDF-1 receptor, coreceptor for X4 HIV-1
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.
Gene ID: 7852 View all products for this Gene ID
UniProt: View information about CD184 on UniProt.org

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