Brilliant Violet 510™ anti-human CD184 (CXCR-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2508	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Chimpanzee, Sooty Mangabey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

CP-MAC-infected Sup-T1 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections¹¹, immunocytochemistry³, immunofluorescence microscopy^2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-1^4,5. Clone 12G5 may not be suitable for Western blotting.¹⁰ The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).

Application References (PubMed link indicates BioLegend citation)

McKnight A, et al. 1997. J. Virol. 71:1692.
Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
Ullrich CK, et al. 2000. Blood 96:1438. (Block)
Murga M, et al. 2005. Blood 105:1992. (IF)
Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
Isnardi I, et al. 2010. Blood 115:5026. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Fischer T, et al. 2008. PLoS One 3:e4069.
Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)

RRID

AB_2810460 (BioLegend Cat. No. 306535) AB_2810461 (BioLegend Cat. No. 306536)

Antigen Details

Structure: Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD
Distribution: T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells
Function: B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
Ligand/Receptor: SDF-1 receptor, coreceptor for X4 HIV-1
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.
Gene ID: 7852 View all products for this Gene ID
UniProt: View information about CD184 on UniProt.org

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