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Brilliant Violet 421™ anti-human CD184 (CXCR
产品名称：
Brilliant Violet 421™ anti-human CD184 (CXCR
产品类别：
抗体
产品编号：
306517
产品应用：
306517
[价格]
规格 价格 库存
25tests ￥ 2280 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CP-MAC-infected Sup-T1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).

Application References

(PubMed link indicates BioLegend citation)
  1. McKnight A, et al. 1997. J. Virol. 71:1692.
  2. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
  3. Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
  4. Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
  5. Ullrich CK, et al. 2000. Blood 96:1438. (Block)
  6. Murga M, et al. 2005. Blood 105:1992. (IF)
  7. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
  8. Isnardi I, et al. 2010. Blood 115:5026. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Fischer T, et al. 2008. PLoS One 3:e4069.
  11. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)
Product Citations
  1. Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed
  2. Saxena S, et al. 2016. Stem Cell Reports. 6: 692-703. PubMed
  3. Terenzi DC, et al. 2021. STAR Protoc. 2:100311. PubMed
  4. Tabler C, et al. 2014. J Virol. 88:4976. PubMed
  5. Mair B et al. 2019. Cell reports. 27(2):599-615 . PubMed
  6. Smulski C, et al. 2017. Cell Rep. 18(9):2189-2202. PubMed
  7. Xu J, et al. 2017. Clin Cancer Res. 23:4482. PubMed
  8. Garcia F, et al. 2020. Sci Rep. 10:14262. PubMed
  9. Demberg T, et al. 2014. Clin Immunol. 153:264. PubMed
  10. Gage BK, et al. 2020. Cell Stem Cell. 27(2):254-269.e9. PubMed
RRID
AB_10901163 (BioLegend Cat. No. 306517) AB_11146018 (BioLegend Cat. No. 306518)

Antigen Details

Structure
Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD
Distribution

T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells

Function
B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
Ligand/Receptor
SDF-1 receptor, coreceptor for X4 HIV-1
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.

Gene ID
7852 View all products for this Gene ID
UniProt
View information about CD184 on UniProt.org

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