Purified anti-β Catenin 1 (CTNNB1) Antibody
- 产品名称:
- Purified anti-β Catenin 1 (CTNNB1) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 844601
- 产品应用:
- 844601
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant β-catenin (N-terminal fragment) fused to MBP.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICFC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10 ?g/mL is suggested. For intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunfluorescence microscopy4 and immunohistochemical staining3.
For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 844603) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
- Application References
(PubMed link indicates BioLegend citation) -
- Sacco P, et al. 1995. J. Biol. Chem. 270:20201. (WB)
- Johnson KR, et al. 1993. Exp. Cell Res. 207:252.
- Gupta K, et al. 2012. J. Ped. Hem. Onc. 34:320. (IHC-P)
- Radice G, et al. 1997. Dev. Bio. 181:64. (IHC-P)
- Product Citations
-
- Davies SP, et al. 2019. Cell Rep. 29:1610. PubMed
- Huang F, et al. 2018. Oncol Rep. 39:809. PubMed
- Nanna AR, et al. 2020. Nucleic Acids Res. 48:5281. PubMed
- RRID
- AB_2750041 (BioLegend Cat. No. 844601) AB_2565803 (BioLegend Cat. No. 844602)
- Structure
- 88 kD armadillo repeat containing protein with an unstructured N and C terminal.
- Distribution
-
β-catenin localizes to cell-cell contacts (adherens junctions) and the cell membrane as well as the cytoplasmic pool of protein. β-catenin can also translocate to the nucleus and is in interphase found in puncta at the proximal end of centrioles. In mitosis, it localizes to the centrosomes at the spindle.
- Function
- Wnt signaling pathway, regulation of cell-cell adhesion, neurogenesis, transcription, transcription regulation, early embryonic patterning, asymmetric cell division, stem cell renewal, and the epithelial to mesenchymal transition.
- Interaction
- Wnt signaling pathway, apoptotic cleavage of cellular proteins, and EphB-EphrinB signaling.
- Biology Area
- Cell Adhesion, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Hirano S, et al. 2012. Physiol. Rev. 92:597.
- Gene ID
- 1499 View all products for this Gene ID
- UniProt
- View information about beta Catenin 1 on UniProt.org