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Purified anti-β Catenin 1 (CTNNB1) Antibody
产品名称:
Purified anti-β Catenin 1 (CTNNB1) Antibody
产品类别:
抗体
产品编号:
844601
产品应用:
844601
[价格]
规格 价格 库存
25µg ¥ 1344 1

产品详情

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant β-catenin (N-terminal fragment) fused to MBP.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 ?g/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10 ?g/mL is suggested. For intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunfluorescence microscopy4 and immunohistochemical staining3.

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 844603) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Sacco P, et al. 1995. J. Biol. Chem. 270:20201. (WB)
  2. Johnson KR, et al. 1993. Exp. Cell Res. 207:252.
  3. Gupta K, et al. 2012. J. Ped. Hem. Onc. 34:320. (IHC-P)
  4. Radice G, et al. 1997. Dev. Bio. 181:64. (IHC-P)
Product Citations
  1. Davies SP, et al. 2019. Cell Rep. 29:1610. PubMed
  2. Huang F, et al. 2018. Oncol Rep. 39:809. PubMed
  3. Nanna AR, et al. 2020. Nucleic Acids Res. 48:5281. PubMed
RRID
AB_2750041 (BioLegend Cat. No. 844601) AB_2565803 (BioLegend Cat. No. 844602)

Antigen Details

Structure
88 kD armadillo repeat containing protein with an unstructured N and C terminal.
Distribution

β-catenin localizes to cell-cell contacts (adherens junctions) and the cell membrane as well as the cytoplasmic pool of protein. β-catenin can also translocate to the nucleus and is in interphase found in puncta at the proximal end of centrioles. In mitosis, it localizes to the centrosomes at the spindle.

Function
Wnt signaling pathway, regulation of cell-cell adhesion, neurogenesis, transcription, transcription regulation, early embryonic patterning, asymmetric cell division, stem cell renewal, and the epithelial to mesenchymal transition.
Interaction
Wnt signaling pathway, apoptotic cleavage of cellular proteins, and EphB-EphrinB signaling.
Biology Area
Cell Adhesion, Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Adhesion Molecules
Antigen References

1. Hirano S, et al. 2012. Physiol. Rev. 92:597.

Gene ID
1499 View all products for this Gene ID
UniProt
View information about beta Catenin 1 on UniProt.org

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